Amid many biomedical researches, the hu-mice model can extremely facilitate the study of hematopoietic malignancies such as acute myeloid leukemia (AML). This progression in the hu-mice model can be used as an effective tool in understanding normal human hematopoiesis including immune cells in vivo ( Lubin et al., 1991 Lapidot et al., 1992 Huntington et al., 2009). Since NOD/SCID mutated mice from the CB17-scid mice can allow superior engraftment of human cells ( Shultz et al., 1995) than nude and SCID mice, the hu-mice model using NOD/SCID and Rag2 nullIL2Rγ null strain mice has been used recently due to loss of murine immune network in immunology fields ( Goldman et al., 1998 Kollet et al., 2000 Ishikawa et al., 2005). Because these mice display a long life span, impaired innate/ adaptive immunity, and engage in the development of hematopoietic lineage cells of donors, these immunodeficient mice are regarded as having a strong strain to sustain human cell engraftment. The hu-mice model is mainly divided into four classes including NOD/ LtSz- scid IL2Rγ null (termed NSG, generated from Jackson Lab), NODShi.Cg-PrkdcscidIL2Rγ trunc (termed NOG, generated from Central Institute for Experimental Animals), BALB/c-Rag2 nullIL2Rγ null (termed BRG, generated from Yale Univ.) and Stock-H2 d-Rag2 nullIL2Rγ null (termed H2 dRG, generated from Pasteur Institute) ( Shultz et al., 2007). An advanced hu-mice model has already been developed by increasing the defected function of immune cells and hu-mice with impaired natural killer cells is an emerging innovative model to study human diseases. The humanized mice (hu-mice) model is commonly reconstituted with human blood-derived CD34 + stem cells using immune compromised mice such as SCID ( Bosma et al., 1983) and Rag null strain mice ( Mombaerts et al., 1992). Keywords : Humanized, Mice model, Acute myeloid leukemia In this review, we briefly described the history of development on immunodeficient SCID strain mice for hu-and hu-leukemic-mice model for immunologic and tumor microenviromental study while inferring the potential benefits of hu-leukemic-mice in cancer biology. Also, an advanced humanized leukemic mice (hu-leukemic-mice) model has been developed by improving immunodeficient mice. Although the hu-mice model does not completely recapitulate human condition, it is a key methodological factor in studying human hematological malignancies with impaired immune cells. Because generated hu-mice harboring a human immune system have displayed phenotype of human CD45 + hematopoietic cells and when played partly with functional immune network, it could be used to evaluate human cell properties in vivo. Moreover, hu-mice models that simply received human CD34 + blood cells and tissue transplants are also overwhelmingly useful in immunology and stem cell biology.

In general, Prkdc scid leakiness is low on the NOD/ShiLtSz genetic background.A humanized mice (hu-mice) model is extremely valuable to verify human cell activity in vivo condition and is regarded as an important tool in examining multimodal therapies and drug screening in tumor biology. Prkdc scid mice accept allogeneic and xenogeneic grafts making them an ideal model for cell transfer experiments. Mice homozygous for the severe combined immune deficiency spontaneous mutation Prkdc scid, commonly referred to as scid, are characterized by an absence of functional T cells and B cells, lymphopenia, hypogammaglobulinemia, and a normal hematopoietic microenvironment.

What are nod scid mice